Fig. 1.

Expression of secreted Fn is significantly suppressed in macrophages after S. aureus infection. The levels of Fn secreted by THP-1-derived microphages (Mφ), monocytes (Mn), hepatocytes (HepG2), or alveolar basal epithelial cells (A549) after (a) 2 h and (b) 24 h of S. aureus infection were determined and the representative Western blot images are shown. The relative densitometric quantification of above results is shown in (c) and (d), respectively. The numbers ‘12598’ and ‘1221’ represent the S. aureus strains ATCC 12598 and SJC1221 (FnBP deficient), respectively. Other abbreviations: 12,598 dead, heat-killed strain 12,598; 12,598 CM, filtered culture medium containing bacterial exotoxins. LPS was used as an experimental control. The fold change of each sample was compared with untreated cells for each cell type. * P < 0.05