FIG. 7.

Minichromosome mitotic stability assay. The 10.6-kb plasmid minichromosome pMW29, which contains CEN4 and the selectable marker URA3, was introduced into the test strains by transformation. Transformant colonies were grown for 8 to 10 generations in nonselective medium, and the fraction of plasmid-containing cells was determined by plating identical aliquots on selective and nonselective medium. Five colonies were scored for each strain, except for the cse4Δ129 strain for which 10 colonies were scored. The means are plotted, with error bars showing the standard errors of the means. Statistical significance of the differences between the mean of galWT and all others was assessed by Bonferroni's multiple comparison test, computed using Prism 4 statistics software (GraphPad). WT, wild-type.