TABLE 1.
S. cerevisiae strains used in this study
| Strain | Genotypea | Reference or source |
|---|---|---|
| KC100 | MATα ade2-101 his3-11,15 leu2-3 lys2-801 trp1Δ901 ura3-52 cse4::HIS3[pCL1] | 22 |
| ZMY60 | MATaade2 trp1-1 ura3-52 leu2::PET56 | 38 |
| YC405 | MATaade2 trp1-1 ura3-52 leu2::PET56 HA-tagged CSE4 dgrn::TRP1 | This study |
| BY4741 | MATahis3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | 5 |
| BY4734 | MATahis3Δ200 leu2Δ0 met15Δ0 ura3Δ0 trp1Δ63 | 5 |
| 24898 | MATa/MATα his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 met15Δ0/MET15 lys2Δ0/LYS2 ura3Δ0/ura3Δ0 cse4Δ::kanMX4/CSE4 | SGDPb |
| R332-5C | MATahis3Δ leu2Δ0 ura3Δ0 trp1Δ63 cse4Δ::kanMX4[pRB163] | This study |
| R332-4B | MATα his3Δ leu2Δ0 ura3Δ0 trp1Δ63 met15Δ0 lys2Δ0 | This study |
| R358 | MATa/MATα his3Δ/his3Δ leu2Δ0/leu2Δ0 met15Δ0/MET15 lys2Δ0/LYS2 ura3Δ0/ura3Δ0 trp1Δ63/trp1Δ63 cse4Δ::kanMX4/CSE4 | This study |
| R365 | MATa/MATα his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 met15Δ0/MET15 lys2Δ0/LYS2 ura3Δ0/ura3Δ0 trp1Δ63/trp1Δ63 gal-cse4Δ129::TRP1/CSE4 | This study |
| R365-5B | MATahis3Δ leu2Δ0 met15Δ0 lys2Δ0 ura3Δ0 trp1Δ63 gal-cse4Δ129::TRP1 | This study |
| R363-1D | MATahis3Δ leu2Δ0 met15Δ0 lys2Δ0 ura3Δ0 trp1Δ63 gal-CSE4::TRP1 | This study |
| R386-1C | MATα his3Δ leu2Δ0 ura3Δ0 trp1 Δ63 met15Δ0 lys2Δ0 [CFIII(D8B.d.R332-1C)MET15] | This study |
| R367-3A | MATahis3Δ leu2Δ0 met15Δ0 lys2Δ0 ura3Δ0 trp1Δ63 gal-cse4Δ129::TRP1 [CFIII(D8B.d.R332-1C)MET15] | This study |
| R368-2D | MATahis3Δ leu2Δ0 met15Δ0 lys2Δ0 ura3Δ0 trp1Δ63 gal-CSE4::TRP1 [CFIII(D8B.d.R332-1C)MET15] | This study |
| R373-2C | MATahis3Δ leu2Δ0 met15Δ0 lys2Δ0 ura3Δ0 trp1Δ63 | This study |
| 3677 | BY4741 mcm21Δ::kanMX4 | SGDP |
| 2613 | BY4741 mcm22Δ::kanMX4 | SGDP |