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. 2017 Apr 13;16:78. doi: 10.1186/s12943-017-0639-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — The effects of DC-SIGNR knockdown on gastric cancer liver metastasis cascade in vivo. a images of GFP signals of mice 15 days after subcutaneous injection with different concentrations of BGC823 cells infected negative control (left panel). The relationship between tumour volume and fluorescence intensity (right panel). b representative images of GFP signals of mice in each group after intrasplenic injection with 5 × 10⁶ BGC823 cells after knockdown DC-SIGNR and photographs of tumours after dissection. c average volume of tumours on liver and spleen derived from BGC823 cells after infected DC-SIGNR and negative control (left panel) and average volume of tumours on spleen derived from BGC823 cells after infected DC-SIGNR and negative control (right panel). d H&E, CK7, CK20 and Ki-67 staining of sections from liver tumours and spleen tumours were performed (scale bar = 50 μm)