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. 2004 Dec;3(6):1609–1618. doi: 10.1128/EC.3.6.1609-1618.2004

FIG. 7.

FIG. 7.

FM4-64 staining in ESCRT-III mutants. Wild-type (DAY286), snf7Δ/Δ (DAY534), vps4Δ/Δ (DAY537), and bro1Δ/Δ (DAY653) strains were incubated 30 min on ice in M199 medium (pH 8) containing 16 mM FM4-64 (time = 0). Cells were then pelleted, washed, and resuspended in warm M199 medium (pH 8) lacking FM4-64 (time = postincubation). Cells were incubated at 37°C, and at 45-, 90-, and 150-min intervals aliquots were taken and transferred to ice-cold tubes containing sodium azide and sodium fluoride. Photographs are representative of three independent samples. pi, postincubation.