Fig. 1.

Specificity of nested PCR. N₁, serum from a non-infected water buffalo; N₂, DBFP from a non-infected water buffalo; N₃, serum from a non-infected goat; N₄, DBFP from a non-infected goat; B, PBS; M, molecular marker; Lanes 1–5: (1) serum from water buffalo assayed on day 3 post-infection; (2) DBFP from water buffalo assayed on day 3 post-infection; (3) serum from goat assayed on day 3 post-infection; (4) DBFP from goat assayed on day 3 post-infection; (5) DNA from an adult S. japonicum worm