. 2017 Apr 13;10:91. doi: 10.1186/s13068-017-0779-z

Table 2.

Various fatty acid compositions and contents of the transformants under conditions of heat shock for 30 min

Fatty acids	CC-849	aRP1.1	aRP1.2	aRP1.3	aRP1.4	aRP2.1	aRP2.2	aRP2.3
C12:0	0.049 ± 0.001	0.083 ± 0.002	0.083 ± 0.003	0.079 ± 0.001	0.085 ± 0.002	0.083 ± 0.003	0.085 ± 0.004	0.086 ± 0.001
C14:0	0.373 ± 0.028	0.467 ± 0.009	0.452 ± 0.004	0.44 ± 0.015	0.502 ± 0.007	0.416 ± 0.055	0.431 ± 0.009	0.435 ± 0.007
C16:0	17.646 ± 0.179	21.968 ± 0.931^b	23.709 ± 1.406^b	21.003 ± 0.758^b	23.445 ± 0.293^b	20.94 ± 0.719^b	22.76 ± 0.406^b	21.256 ± 1.432^b
C16:1	2.666 ± 0.234	3.032 ± 0.145^a	3.62 ± 0.072^b	3.364 ± 0.038^b	3.353 ± 0.044^b	2.386 ± 0.293	3.092 ± 0.045^a	2.789 ± 0.148
C18:0	3.37 ± 0.272	3.748 ± 0.114^a	3.563 ± 0.023	3.126 ± 0.067	3.487 ± 0.02	3.51 ± 0.177	3.338 ± 0.036	3.059 ± 0.141
C16:4	20.756 ± 1.093	24.823 ± 0.314^b	28.023 ± 1.839^b	28.09 ± 0.848^b	28.529 ± 0.146^b	23.025 ± 0.781^a	24.66 ± 1.453^b	24.532 ± 0.254^b
C18:1 t	2.393 ± 0.387	1.889 ± 0.246	2.625 ± 0.112	1.591 ± 0.06^b	2.133 ± 0.183	1.958 ± 0.589	2.354 ± 0.05	2.011 ± 0.447
C18:2 t	9.163 ± 0.516	7.913 ± 0.555^a	6.73 ± 0.123^b	4.806 ± 0.261^b	9.775 ± 0.354	7.605 ± 1.309	11.37 ± 0.673^b	10.042 ± 1.465
C18:3	6.651 ± 0.565	7.272 ± 0.3	7.352 ± 0.123	7.06 ± 0.116	8.598 ± 0.273^b	8.503 ± 0.591^b	7.663 ± 0.365^a	7.244 ± 0.289
C18:3n3	28.757 ± 1.697	33.663 ± 0.646^b	36.882 ± 1.682^b	35.235 ± 1.615^b	39.886 ± 0.278^b	31.335 ± 1.129^a	35.924 ± 1.12^b	35.765 ± 0.892^b
C20:1	1.475 ± 0.023	1.483 ± 0.023	1.493 ± 0.038	1.656 ± 0.063^b	1.663 ± 0.034^b	1.516 ± 0.117	1.376 ± 0.096	1.354 ± 0.055^b
C20:3	0.053 ± 0.009	0.093 ± 0.003^b	0.096 ± 0.008^b	0.095 ± 0.002^b	0.093 ± 0.003^b	0.098 ± 0.009^b	0.097 ± 0.005^b	0.095 ± 0.005^b
C20:4	0.071 ± 0.002	0.105 ± 0.001^b	0.107 ± 0.004^b	0.106 ± 0.003^b	0.119 ± 0.003^b	0.111 ± 0.003^b	0.118 ± 0.003^b	0.119 ± 0.006^b
C22:2	0.042 ± 0.003	0.081 ± 0.002^b	0.084 ± 0.005^b	0.079 ± 0.001^b	0.083 ± 0.004^b	0.098 ± 0.017^b	0.085 ± 0.005^b	0.082 ± 0.003^b
C24:0	0.215 ± 0.003	0.381 ± 0.013^b	0.382 ± 0.009^b	0.339 ± 0.064^b	0.375 ± 0.016^b	0.36 ± 0.012^b	0.363 ± 0.006^b	0.299 ± 0.063^a
C22:6	0.074 ± 0.005	0.145 ± 0.002^b	0.158 ± 0.015^b	0.142 ± 0.001^b	0.142 ± 0.001^b	0.143 ± 0.004^b	0.142 ± 0.002^b	0.144 ± 0.005^b
TFA	93.754 ± 3.01	107.14 ± 3.306^b	119.21 ± 6.55^b	107.21 ± 3.7^b	122.27 ± 0.86^b	102.1 ± 1.15^b	113.86 ± 3.41^b	109.312 ± 4.99^b

The data represent the means ± SD of three replicate experiments and were analyzed by Student’s t-test (n = 3)

^aP <0.05; ^bP <0.01. aRP1.1, aRP1.2, aRP1.3, aRP1.4 is transformed amicroRNA-PEPC1 into C. reinhardtii; aRP2.1, aRP2.2, aRP2.3 is transformed amicroRNA-PEPC2 into C. reinhardtii