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AQR1 is required for Hom excretion. Cell suspensions of wild-type (23344c), gap1 agp1 gnp1 (JA248), and gap1 agp1 gnp1 aqr1 (IVU271) strains transformed with the pMACR7 plasmid (HOM3-R) were dropped over a lawn of Hom (hom3, strain Σ-A3-hu) auxotroph mutants and the petri dishes were incubated for 4 days at 29°C. Proline was the sole source of nitrogen in the medium.
