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. 2017 Apr 13;14:78. doi: 10.1186/s12985-017-0748-y

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Identification of recombinant DEV CHv BAC colonies. a Purification and enrichment of BAC-recombinant virus. A: The 3rd passage of BAC-recombinant DEV after infection. B-I: Purification and enrichment of BAC-recombinant DEV by eight round of fluorescence plaque selection. b Orientation analysis of pBAC-DEV digested b EcoR I. c Real Gel analysis of pBAC-DEV digested by EcoR I. d Identification of pBAC-DEV. Lane 1–14: PCR product of DEV genes in table 2; Lane 15–17: PCR product of inserting exogenous gene EGFP, repA and sopB