Table 1.

Primers used in this paper

NO.	Primers	Sequence (5’-3’)	Product
1	TKA-HOMO-for	gaattcatgcttgccatcataaccgtattctc	TK left homology arm
	TKA-HOMO-rev	tctagaataacttcgtataatgtatgctatacgaagttatcacctcgagcttttctttcctgtg
2	TKB-HOMO-for	gcatgcacatagcaacaactgacgcaaaagc	TK right homology arm
	TKB-HOMO-rev	aagctttcccagaaagctcgcctaggtcctc
3	EGFP-for	tctagatagttattaatagtaatcaattacg	EGFP
	EGFP-rev	gtcgacatgcagtgaaaaaaatgct
4	sopB-for	attcgttaattgcgcgcgtagg	sopB
	sopB-rev	gaatattcaggccagttatgct
5	repA-for	catggcggaaacagcggttatc	repA
	repA-rev	atgtatgagaggcgcattggag
6	TK-for	cgcggatcccactgaatgtcactgc	TK
	TK-rev	cccaagctttcaattaattgtcatctcggt
7	ΔUL55-KanR-for	gaaaggcggttggaataagaggaacgaggcggtagacgtgaccgacaacagtgtaggctggagctgcttc	KanR gene flanked by homology arms of UL55
	ΔUL55-KanR-rev	tttcttatggttttaataaaacgctttattacattgtagtgtaacaagaccatatgaatatcctccttag
8	ΔUL55/ΔUL55R-for	tgcaaattagtgggaggtacg	ΔUL55/ΔUL55R identification product
	ΔUL55/ΔUL55R-rev	cccaaataccctgttagtagctt
9	ΔUL55R-UL55-for	atggccgacgcgaaggcggt	UL55 fragment with left homology arm of UL55
	ΔUL55R-UL55-rev	gaagcagctccagcctacactcatacattagctttgtg
10	ΔUL55R-KanR-for	cacaaagctaatgtatgagtgtaggctggagctgcttc	UL55 fragment with right homology arm of UL55
	ΔUL55R-KanR-rev	tttcttatggttttaataaaacgctttattacattgtagtgtaacaagaccatatgaatatcctccttag

Italic: Complementary sequence for overlap PCR