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. 2017 Feb 8;27(2):933–949. doi: 10.1093/cercor/bhx016

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© The Author 2017. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Sert^−/− rats show a reduced number of perisomatic inhibitory boutons onto excitatory LIV neurons. (A) Fluorescent photomicrographs of LIV excitatory neurons (NeuN⁺/Gad67⁻) used for quantification of perisomatic GAD67 puncta/µm in Sert^+/+ and Sert^−/− rats. White arrows mark puncta onto a representative soma of a pyramidal cell. (B) The number of GAD67-positive perisomatic varicosities was normalized against the diameter of each presumed excitatory neuron. Histogram shows the plot of mean number of perisomatic boutons per cell (normalized to cell diameter, puncta/µm) in Sert^+/+ (n = 82) and Sert^−/− (n = 91) neurons. (C) Top, example voltage clamp trace of mEPSCs recorded in excitatory LIV neurons of both Sert^+/+ and Sert^−/− rats at V_h = −70 mV. Bottom, cumulative probability distributions and histograms of amplitude and frequency of mEPSCs in Sert^+/+ (n = 12) and Sert^−/− (n = 11). (D) Top, example voltage clamp trace of mIPSCs recorded in excitatory LIV neurons of both Sert^+/+ and Sert^−/− rats at V_h = +10 mV. Bottom, cumulative probability distributions and histograms of amplitude and frequency of mIPSCs in Sert^+/+ (n = 17) and Sert^−/− rats (n = 16). (E) Left, example voltage clamp trace of evoked IPSCs onto LIV excitatory neurons within a barrel at different ISI (50, 100, 200, and 500 ms) at V_h = +10 mV in the presence of AMPA/NMDA blockers. Right, line plot depicting PPR Amp2/Amp1 at varying ISIs in Sert^+/+ (n = 8) and Sert^−/− rats (n = 9). Data are represented as mean ± SEM, *P < 0.05 and ***P < 0.001 (t-test).