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. 2017 Feb 8;27(2):933–949. doi: 10.1093/cercor/bhx016

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© The Author 2017. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Reduced inhibitory drive in Sert^−/− rats is associated with a decrease in GABAA α1 subunit expression as well as a reduced KCC2 chloride extruder protein expression within S1. (A) Western blot analysis of GABAA α1 subunit expression in S1 total protein lysates of Sert^+/+ (n = 4) and Sert^−/− rats (n = 4). Left, loading control. Right, western blot and histogram showing total GABAA α1 receptor subunit expression normalized to Sert^+/+. (B) Western blot analysis of GABAA α2 receptor subunit expression in S1 total protein lysates of Sert^+/+ (n = 4) and Sert^−/− rats (n = 4). Left, loading control. Right, western blot and histogram showing total GABAA α2 subunit expression normalized to Sert^+/+. (C) Left, surface and internal NKCC1 protein levels were determined by BS³ cross-linking method using S1 protein lysates of Sert^+/+ and Sert^−/− rats with non-crosslinked control (Ctrl). Right, histograms showing quantification of total NKCC1 expression normalized to γ-tubulin and surface NKCC1 expression normalized to internal expression in Sert^+/+ (n = 12) and Sert^−/− (n = 12) rats. (D) Left, surface and internal KCC2 protein levels were determined by BS³ cross-linking method using S1 protein lysates of Sert^+/+ and Sert^−/− rats with non-crosslinked control (Ctrl). Right, histograms showing quantification of total KCC2 expression normalized to γ-tubulin and surface KCC2 expression normalized to internal expression in Sert^+/+ (n = 12) and Sert^−/− rats (n = 12). All data are presented as mean ± SEM, *P < 0.05 and **P < 0.01 (t-test).