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Confirmation of microarray results by qPCR
| Results | Lgals | Fcgr2b | Gpnmb | C3 | Atf3 | Ptprc | Cd163 | Nrg1 | Cd11b |
|---|---|---|---|---|---|---|---|---|---|
| ΔΔCt M v Y | 1.62 | 1.68 | 1.76 | 1.42 | 0.94 | 0.99 | 0.78 | −0.79 | 0.87 |
| qPCR Fold M/Y | 3.08 | 3.20 | 3.40 | 2.68 | 1.92 | 1.99 | 1.72 | 0.58 | 1.83 |
| Microarray M/Y | 2.44 | 2.89 | 3.14 | 2.40 | 1.73 | 1.84 | 1.59 | 0.46 | N/A |
| Adjusted p-value | 0.004 | 0.003 | 0.006 | 0.010 | 0.004 | 0.001 | 0.001 | 0.056 | 1.0E-07 |
cDNA was synthesized from the same RNA samples analyzed in the microarrays. ΔΔCt values are expressed as described in Methods
