Fig. 1.

Isolation of GFP-positive NCCs. a Wholemount E9.5 Wnt1Cre; Z/EG embryos immunolabelled for GFP identifies NCCs in the r1-2 (r2) and r4 migratory streams, and additional Wnt1 expression domains within the mid brain (mb). b Sox10 in situ hybridisation of wild type E9.5 embryos. c-f Schematic of work flow for isolation of GFP-positive NCCs from Wnt1Cre; Z/EG embryos. c Wnt1Cre; Z/EG embryos were dissected into r1-r2, r4 and trunk regions and dissociated in tryple express (d). e Trunk NCCs were used to set GFP gates for FACS. f GFP-positive NCCs were collected for each population and RNA extracted (g). h qRT-PCR was performed for Sox10 to confirm NCC isolation. ov, otic vesicle; e, eye. Scale Bars = 500um