Fig. 5.

A-type Plexins are expressed within cranial NCCs. a-p Wholemount in situ hybridisation of wild type E9.5 embryos (a, e, i, m) were sectioned longitudinally (b-c, g-f, j-k, n-o) and immunolabelled for NGFR (d, h, l, p) to identify migrating NCCs. a-e PlxnA1 is diffusely expressed throughout the mesenchyme of the frontonasal process, premaxilla and in the neural tube. f-i PlxnA2 is expressed in the frontonasal process, premaxilla, diencephalon, rhombomeres and NCCs within the r1-r2 and r4 migratory streams. j-m PlxnA3 is expressed in the frontonasal process, premaxilla and facioacoustic ganglia. n-q PlxnA4 is expressed diffusely throughout the frontonasal process, premaxilla, PA1 and NCCs within the r4 migratory stream. ov, otic vesicle; e, eye. Scale Bars (a, e, i, m) = 500um and (b, f, j, n) = 100um