Skip to main content
. Author manuscript; available in PMC: 2017 Dec 15.
Published in final edited form as: Cell. 2016 Dec 15;167(7):1705–1718.e13. doi: 10.1016/j.cell.2016.11.055

Figure 4. The NPC Connects Mitochondria to Regulation of mTORC1 in Metformin Action.

Figure 4

(A and B) NPC deficiency antagonizes the effect of rotenone (5 µM), but not rapamycin (100 µM), on CeACAD10 induction. n = 3 biological replicates; **p < 0.01, by two-way ANOVA.

(C and D) shRNA knockdown of NPC components in HEK293E cells prevents metformin (8 mM) and rotenone (40 µM) from reducing S6 protein phosphorylation, but not rapamycin (40 nM). Blots shown are representative of two biological replicates.