(A and B) NPC deficiency antagonizes the effect of rotenone (5 µM), but not rapamycin (100 µM), on CeACAD10 induction. n = 3 biological replicates; **p < 0.01, by two-way ANOVA.
(C and D) shRNA knockdown of NPC components in HEK293E cells prevents metformin (8 mM) and rotenone (40 µM) from reducing S6 protein phosphorylation, but not rapamycin (40 nM). Blots shown are representative of two biological replicates.