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. 2017 Apr 13;8:579. doi: 10.3389/fpls.2017.00579

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Copyright © 2017 Sen Gupta, McPhee and Kumar.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Dissociation curve for Ferritin-1 primer pairs. Derivative plotted in Y-axis is the negative of the rate of change in fluorescence as a fraction of temperature and temperature is plotted on the X-axis. (B) Dissociation curve for BHLH-1 primer pairs. Derivative plotted in Y-axis is the negative of the rate of change in fluorescence as a fraction of temperature and temperature is plotted on the X-axis. (C). Dissociation curve for IRT-1 primer pairs. Derivative plotted in Y-axis is the negative of the rate of change in fluorescence as a fraction of temperature and temperature is plotted on the X-axis.