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Sequence and Tm (melting temperature) for primers designed based on the CDC Redberry contig (LcRBContig00605) for the Ferritin-1 gene in lentil.
| Primer name | Forward sequence (5′–3′) | Reverse sequence (5′–3′) | Tm (°C) |
|---|---|---|---|
| FerrClo1 | TGCTGATAAGGGTGATGCGCT | GGCTTCCACCTGTTCACCCA | 64 |
| FerrClo2 | AACCTGCACAGTGTTGCCTC | AGTGCCAGACACCATGTCCT | 62 |
| FerrClo3 | GTTGCGCTGAAAGGTCTTGCT | GCCAAGTGCACATCACCAGT | 62 |
| FerrClo4 | ACGTTGCGCTGAAAGGTCTTG | TGCCAAGTGCACATCACCAG | 62 |
| FerrClo5 | CTGGTGATGTGCACTTGGCA | GCTGCAGCTGCTTCCTCACT | 62 |
