Table 3.

Amplification statistics for one Ferritin-1, one BHLH-1, one IRT-1 gene specific primer pairs, and one primer pair for each reference gene (GADPH, Actin).

Gene	Forward sequence	Reverse sequence	Tm (°C)	Size (bp)	Slope	R2	E	Reference
Ferritin-1	AGATATCCGAGTATGTTGCTCAG	AAGATGCACGAATGAAGCAGAAA	61	84	–3.32	0.9968	100.07	Current work
IRT-1	GTCGCTGTTTTGCTAGGTGC	GTGAGCTTCTCCTCTTCCCT	61	159	–3.12	0.9954	109.18	Current work
BHLH-1	TTATTAGGGTTAGACTCAACGCA	TTGCGATCTTTGGTTCCCA	59	74	–0.02	0.0034	6.55e+42	Sen Gupta et al., 2016
GADPH	TGGGCGAAAACTCCACTTTG	GAATTGCTGCAGCCTTGTGA	60	57	–3.15	0.9954	107.71	Saha and Vandemark, 2012
Actin	CCAAATCATGTTTGAGGCTTTTAA	GTGAAAGAACGGCCTGAATAGC	60	64	–3.55	0.9972	91.25	Saha and Vandemark, 2012

Here, T_m = melting temperature, Size = amplicon length, Slope = slope of the trend line in amplification efficiency graph, R²= regression coefficient, E = amplification efficiency.