Table 1.
Primer sequences for real-time PCR.
| Gene | Sequence |
|---|---|
| mRANTES | Fwd: CACCACTCCCTGCTGCTTTG |
| Rev: CGACTGCAAGATTGGAGCACTT | |
| mTNF-α | Fwd: ACGGCATGGATCTCAAAGAC |
| Rev: GTGGGTGAGGAGCACGTAGT | |
| mIL-1β | Fwd: GCCCATCCTCTGTGACTCAT |
| Rev: AGGCCACAGGTATTTTGTCG | |
| mIFN-γ | Fwd: GCTACACACTGCATCTTGGCTTT |
| Rev: AATGACTGTGCCGTGGCAGTAA | |
| hCOX-2 | Fwd: GATACTCAGGCAGAGATGATCTACCC |
| Rev: AGACCAGGCACCAGACCAAAGA | |
| hPGES | Fwd: CCTGGGCTTCGTCTACTCCTTT |
| Rev: CCCACGAGGAAGACCAGGAA | |
| h15-PGDH | Fwd: GAAGGCGGCATCATTATCAATATG |
| Rev: GCTGAGCGTGTGAATCCAACTAT | |
| hEP1 | Fwd: TTGTCGGTATCATGGTGGTG |
| Rev: ATGTACACCCAAGGGTCCAG | |
| hEP2 | Fwd: CCTGCTGCTGCTTCTCATTGTCT |
| Rev: GGCTTCGGCGGTGCAT | |
| hEP3 | Fwd: AAGGCCACGGCATCTCAGT |
| Rev: ACGCACATGATCCCCATAAGC | |
| hEP4 | Fwd: TCCGGCCTCAGCATCATC |
| Rev: GGCTGTAGAAATAGGCATGGTTGA | |
| mGAPDH | Fwd: TGCAGTGGCAAAGTGGAGATT |
| Rev: CGTGAGTGGAGTCATACTGGAACA | |
| hGAPDH | Fwd: GAAAAACCTGCCAAATATGATGACA |
| Rev: TAGCCCAGGATGCCCTTGAG |
m, mouse; h, human; Fwd, forward; Rev, reverse; RANTES, regulated on activation normal T-cell expressed and secreted; TNF-α, tumor necrosis factor-α; IL-1β, interleukin-1β; IFN-γ, interferon-γ; COX, cyclooxygenase; PGES, prostaglandin E synthases; 15-PGDH, 15-hydroxyprostaglandin dehydrogenase; EP, prostaglandin receptor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.