Figure 2.

Confirmation of reduction of Rdh10 mRNA in mice with conditional null allele. (A) Schematic drawing of the method used to generate the Rdh10 conditional null allele (modified from reference (18). (B–E) In situ hybridization of Rdh10 using an Exon2 specific riboprobe. E10.5 and E11.5 control embryos (B and C) compared with the same stage of Cre-ER^T2; Rdh10^flox/flox embryos (D and E) which were administered tamoxifen at E7.5. Red arrowhead indicates the lambdoidal region in B,D; and primitive choanae in C,E. TMX E7.5, tamoxifen was administered at E7.5. E, eye. MXP, maxillary process. MN, mandibular process. MNP, medial nasal process. LNP, lateral nasal process. FL, forelimb. Scale bar = 500 μm in B,D and 200 μm in C,E.