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. 2017 Apr 11;3(4):e00291. doi: 10.1016/j.heliyon.2017.e00291

Fig. 3.

Fig. 3

SDS-PAGE and immunoblot analysis of proteins attached to PHB beads and purified His6-PsaA. A, Comassie blue stained SDS-PAGE gel; B, immunoblot of (A) developed using monoclonal anti-PsaA antibodies. Lane 1, Molecular weight (MW) standard (Novex® Sharp Pre-Stained Protein Standard, Invitrogen); lane 2, purified His6-PsaA (MW: 34 kDa) derived from cells harboring pET14b-his6-psaA; lane 3, PHB beads isolated from cells harboring pET-14b-phaC encoding PhaC (MW: 64.5 kDa); lane 4, PHB beads isolated from cells harboring pET-14b-psaA-phaC encoding PsaA-PhaC fusion protein (MW: 97 kDa). The arrows indicate the protein band of interest with the corresponding theoretical MW.