Fig. 5. Photo-uncaging of 1 is spatially selective and leads to a decrease in IL-12 production in only the irradiated macrophages. BMDM were pre-treated with 1 (3 μM), and the center of the dish was irradiated with UV light (epifluorescence using DAPI filter, 30 s). The BMDM were then activated with LPS (100 ng mL–1). After 6 h, the cells were fixed, and immunofluorescence labeling for IL-12 was performed, followed by imaging with confocal microscopy. Shown are maximum intensity z-projection images of cells at the border of UV irradiation. Dotted line indicates the border region of illumination. First three panels represent monochrome images of non-photoconverted mEos3.2, photoconverted mEos3.2, and IL-12, respectively. Far right panel represents merged image of photoconverted mEos3.2 (red) and IL-12 (cyan). Scale bar: 50 μm.
