Figure 3. IL-6 induces in vitro migration of endothelial cells and fibroblasts.
IL-6 chemotaxis for endothelial cells (ECs), macrophages, smooth muscle cells (SMCs) and fibroblasts was tested by Oris Pro Cell Migration and Invasion assay kit with 50 ng/mL of IL-6 protein or without protein (PBS) (n=8 each group). A) After the assay was performed, the migratory cells were measured using Image Pro software. The assay revealed that IL-6 induces ECs and fibroblast migration but not macrophages and SMCs. B) Representative images of migration assays in each group. White dashed line: pre-migration area (Diameter=2mm). Black dotted line: post-migration area.