. 2015 Mar 16;5:9139. doi: 10.1038/srep09139

Table 1. List of starting materials employed and the types of poly-substituted purines synthesized. Half-inhibitory concentrations (IC₅₀) against the protozoan parasite Trypanosoma brucei presented as mean ± SEM. n = 6.

entry	R¹	R²	R³	“A”% (R¹, R², R³)	“B”% (R²,R³)	IC₅₀ (μM)^a T.brucei
A1	Me	Ph	ⁱPr	16%	traces	69.2 ± 1.8
A2	Et	Me	ⁱPr	29%	traces	137.1 ± 6.9
A3¹	Et	Me	Bu	31%	traces	51.8 ± 3.3
A4	H	Me	ⁱPr	44%	-	14.7 ± 0.6
A5	H	Ph	ⁱPr	38%	-	1 ± 0.1
B1	Et	Ph	ⁱPr	-	40%	52.1 ± 1
B2	Et	Ph	^tBu	-	45%	18.3 ± 0.5
B3	Et	MeOPh	ⁱPr	-	28%	42.7 ± 1
B4	Et	MeOPh	^tBu	-	22%	80.5 ± 3.5
B5	ⁱPr	H	ⁱPr	-	10%	37.5 ± 1.5
B6	ⁱPr	Me	ⁱPr	-	10%	32 ± 1.7
B7	Ph	Me	ⁱPr	-	20%	22.7 ± 1.4
B8	Ph	Et	ⁱPr	-	27%	4.8 ± 0.2
B9	Ph	ⁱPr	ⁱPr	-	22%	12.6 ± 0.6

^aCell viability was assessed by resazurin dye. Half-inhibitory concentration (IC₅₀) values were calculated using GraphPad Prism5 Software and defined as the concentration of purine required to diminish fluorescence output by 50%. n = 6.

Table 1. List of starting materials employed and the types of poly-substituted purines synthesized. Half-inhibitory concentrations (IC50) against the protozoan parasite Trypanosoma brucei presented as mean ± SEM. n = 6.

Table 1. List of starting materials employed and the types of poly-substituted purines synthesized. Half-inhibitory concentrations (IC₅₀) against the protozoan parasite Trypanosoma brucei presented as mean ± SEM. n = 6.