Figure 3. DNA – membrane interaction for different HV-LV protocols for c_DNA = 10 μg/ml and 2 μg/ml.

The average maximal fluorescence intensity FL^TOTO is presented as an average ± standard error; the representative images for HV, HV+LV, LV+HV and LV pulses are shown on top. Please note that the scale of fluorescence intensity in the micrographs is adjusted between Min = 100 and Max = 700 A.U. in images with c_DNA = 10 μg/μl, and between Min = 100 and Max = 300 A.U. for c_DNA = 2 μg/ml. Immediately after the labeled plasmid was added to the cells, different combinations of HV (4 × 200 μs, E_HV = 1.4 kV/cm, 1 Hz) and LV pulses (1 × 100 ms, E_LV = 0.137 kV/cm) were applied.