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. 2017 Apr 13;12(4):e0174969. doi: 10.1371/journal.pone.0174969

Fig 5. Influence of chondrogenic differentiation on FGFR activation.

Fig 5

ATDC5 were cultured for 14 days with insulin. Total protein was then extracted from ATDC5 and subjected to Phospho-Tyrosine Mouse mAb (OUTPUT) or not (INPUT). Antibodies complexes were eluted and resolved by SDS-PAGE with FGFR1, FGFR3, FGFR4 (1/200) or Erk (1/500) antibodies. Erk protein was used as non-tyrosine-phosphorylated protein control.