Table 2. Effect of specific inhibition of FGFRs activation during ATDC5 differentiation process (Day 14).

PD173074	-	+
	Fold expression
Col II	2.5 ± 0.1	2.3 ± 0.19
Col X	2.5 ± 0.35	1.35 ± 0.15 ***
MMP13	1.4 ± 0.15	1.32 ± 0.092
FGFR4	2.12 ± 0.326	1.9 ± 0.14
FGF23	1.2 ± 0.18	1.21 ± 0.11

Total RNA was extracted from ATDC5 cultured in ITS conditions for 14 days treated or not with 0.5 μM PD173074, then reverse transcribed into cDNA and analyzed by real-time PCR. The relative abundance of COL II, COL X, MMP13, FGFR4 and FGF23 was normalized to RPS29 mRNA. Comparison was made by using the ΔΔCt method with the fold value of reference (fold = 1) assigned to D0.

*** p<0,001 vs ITS.

Data are expressed as mean ± SD, n = 3.