Fig 3. Respiration of cortex, liver, soleus muscle and heart of Hdh^Q20 and Hdh^Q111 mice.

High-resolution respirometry in the murine tissues prefrontal cortex, liver, soleus muscle and heart. The white boxes represent Hdh^Q20 control mice versus the gray boxes, which represent the HD mouse model Hdh^Q111. (A) Complex I activity (C_I) is shown in a coupled state determined after addition of ADP. (B) Complex II activity (C_II) was measured in an uncoupled state after addition of rotenone. (C) The maximum OxPhos capacity was measured and (D) the maximum uncoupled capacity was determined after application of FCCP in all four tissues. (E) For murine cortex and liver the oxygen consumption linked to ATP production (O₂ATP) was calculated. n = 6, student’s t-test comparing genotypes, ns = p>0.05, * = p≤0.05, ** = p≤0.01. 95% Confidence interval limits: (B) liver: 14.72–65.71; (D) cortex: 1.20–28.16; liver: 4.99–73.43.; (E) cortex: 0.96–13.67. The Mann-Whitney Rank-Test was used for the following samples: (A): cortex; (B): cortex, m. soleus, heart; (D): m. soleus.