Fig 4. Mitochondrial mass in HdhQ mice in brain, soleus muscle, liver and heart.

(A) The mtDNA copy number was determined by qPCR for the murine cortex, liver, soleus and heart muscle, comparing Hdh^Q20 control mice (white boxes) to Hdh^Q111 HD mice (gray boxes). n = 6–7. (B) The citrate synthase (CS) activity was measured by a spectrophotometric assay. n = 5, student’s t-test comparing genotypes of one tissue, ns = p>0.05. (C) Correlation of mtDNA copy number to the citrate synthase activity, which was determined in triplicates (±SD). Pearson value p≤0.001, R² = 0.5371. The Mann-Whitney Rank-Test was used for the following samples: (B): cortex, liver.