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. 2017 Apr 13;12(4):e0174873. doi: 10.1371/journal.pone.0174873

Fig 3. Relative expression of MRO transcripts was analyzed by qPCR.

Fig 3

(A) Schematic representation of the MRO splice variants and the position of the TaqMan probes. Overall expression detected by the pQ8/9 probe. pQ1/3 spans exons 1–3 and detects the 'b' isoforms (MROb1-4); pQ2/3 spans exons 2/3 and detects isoforms MROa,c,d. The expression of MRO was normalized to 18s RNA and presented as relative a expression ratio (1/ΔCt). (B) MRO transcripts analysis in GCs and CCs from PCOS and control subjects. The overall expression, shown by exon 8/9 was higher in PCOS cells vs that of controls (* in GCs and * in CCs; p ≤0.05). Inverse expression of exon 1/3 in GCs and CCs vs exon 2/3 was detected. (C) Inverse expression of exon 2/3 vs 1/3 in kidney, liver, brain and post-menopausal ovary.