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. 2017 Apr 13;12(4):e0174873. doi: 10.1371/journal.pone.0174873

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© 2017 Kenigsberg et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 6 — Immunofluorescence was performed on non-PCOS (A) and PCOS (B and C) ovarian sections using the FL-248 antibody (4ug/ml). Nuclear staining is apparent in the granulosa cells (magnified images), while the blocking peptide abolished the MRO signal (C). MRO expression was observed in green (Alexa-fluor 488) and nucleus in blue (DAPI). Magnification: x100. Duration of exposure: 2000 milliseconds (non-PCOS) and 1500 milliseconds (PCOS) demonstrating that even with short exposure the fluorescence intensity is higher in PCOS samples.