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The amplification program was as follows: a uracil-DNA glycosylase (UDG) incubation (50°C, 2 min) and initial DNA polymerase enzyme activation (95°C, 10 min), followed by 40 cycles of denaturation (95°C, 15 sec) and annealing ⁄ extension (60°C, 1 min).
| Probe name | Exons span | Cat. # |
|---|---|---|
| pQ-1/3 | exons 1, 3 | Hs00901130_m1 |
| pQ-2/3 | exons 2, 3 | Hs00903498_g |
| pQ-8/9 | exons 8, 9 | Hs00901134_m1 |
| 18sRNA | Hs99999901_s1 |
