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. 2017 Apr 13;12(4):e0175435. doi: 10.1371/journal.pone.0175435

Fig 5. Effects of DEX on the mRNA expression of Tpit, Pitx1, Nur77 and Nurr1 in AtT20 cells.

Fig 5

(A) Time-dependent effect of DEX on Tpit mRNA expression. AtT20 cells were treated with DEX (100 nM) for 1 hr, 3 hrs, 6 hrs, 9 hrs, or 24 hrs. Vehicle control: 0.1% ethanol. Results are expressed as percentages of control (100%). Each point represents mean ± SEM (n = 4). NS denotes “Not Significant.” Two independent experiments were performed with consistent results. (B) Time-dependent effect of DEX on Pitx1 mRNA expression. AtT20 cells were treated with DEX (100 nM) for 1 hr, 3 hrs, 6 hrs, 9 hrs, or 24 hrs. Vehicle control: 0.1% ethanol. Results are expressed as percentages of each control (100%). NS denotes “Not Significant.” Two independent experiments were performed with consistent results. (C) Time-dependent effect of DEX on Nur77 mRNA expression. AtT20 cells were treated with DEX (100 nM) for 1 hr, 3 hrs, 6 hrs, 9 hrs, or 24 hrs. Vehicle control: 0.1% ethanol. Results are expressed as percentages of control (100%). NS denotes “Not Significant.” Two independent experiments were performed with consistent results. (D) Time-dependent effect of DEX on Nurr1 mRNA expression. AtT20 cells were treated with DEX (100 nM) for 1 hr, 3 hrs, 6 hrs, 9 hrs, or 24 hrs. Vehicle control: 0.1% ethanol. Results are expressed as percentages of control (100%). Each point represents mean ± SEM (n = 4). NS denotes “Not Significant.” ***P<0.001 vs control. Two independent experiments were performed with consistent results.