Figure 1.

The hydroalcoholic Achillea wilhelmsii C. Koch extract (HAWE) induced apoptosis in MCF-7 and MDA-Mb-468 cell lines. MCF-7 and MDA-Mb-468 cells were treated with different concentrations of HAWE (0 [control], 12.5, 25, 50, 100 µg/mL). After 48 hours of treatment, (A) MCF-7 and (B) MDA-Mb-468 cells were double stained with Annexin V-fluorescein isothiocyanate and propidium iodide, and the cells were analyzed by flow cytometry. All experiments were performed independently in triplicate per experimental point, and representative results are shown.