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. 2017 Apr 5;13:1744806917701445. doi: 10.1177/1744806917701445

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© The Author(s) 2017

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 2. — The expression of the human proenkephalin (hPPE) gene in engineered hBMSCs (c) was analyzed by RT-PCR two weeks (passage 3) after cell transfer. hBMSCs, human bone marrow stem cells; pBABE, a retroviral vector; pBABE-hBMSCs, the pBABE-hBMSCs group; hPPE-hBMSCs, the hPPE-hBMSCs group. Naive hBMSCs (a) and vector-engineered hBMSCs (b) served as controls, and hPPE RT-PCR products (161 bp) were expressed as the hPPE/GADPH (450 bp, an internal control) ratio. Statistical analysis showed significantly upregulated hPPE expression in hPPE-hBMSCs compared with hBMSCs and pBABE-hBMSCs (P < 0.01). *P < 0.01 versus normal hBMSCs and ^#P < 0.01 versus pBABE-hBMSCs, n = 6.