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. 2017 Apr 5;13:1744806917701445. doi: 10.1177/1744806917701445

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© The Author(s) 2017

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — The different expressions levels of Leu-enkephalin (L-EK) protein were compared between the pBABE-hBMSC group and the hPPE-hBMSC group by immunofluorescence. pBABE, a retroviral vector; hPPE, human proenkephalin; hBMSCs, human bone marrow stem cells. pBABE-hBMSCs, the pBABE-hBMSCs group; hPPE-hBMSCs, the hPPE-hBMSCs group. Blue ﬂuorescence marks the nucleus of the hBMSCs by 4′6-diamidino-2-phenylindole. Red ﬂuorescence marks L-EK protein. Double-labeled cells, with a blue ﬂuorescent nucleus and a red cytoplasm, represented hBMSCs that expressed the L-EK. There were no differences in nuclear staining between the groups. Little L-EK expression was observed in the pBABE-hBMSC group, whereas greater expression was detected in the hPPE-hBMSC group. All images were obtained on a laser scanning confocal microscope (Leica). Scale bars = 50µm. n = 6.