(A) D. antarctica (2n = 26+2B), (B) D. parvula (2n = 26), (C) D. cespitosa (2n = 26), (D) D. cespitosa (2n = 4x = 52), (E) D. danthonioides (2n = 26), (F) D. elongata (2n = 26+2B), (G) D. sukatschewii (2n = 26+M+B). Merged fluorescent images after multicolour FISH with 45S (yellow) and 5S (red) rDNA and sequential rapid GISH with both genomic DNAs: D. cespitosa (green) and D. flexuosa (purple) (A, E, F and G); D. cespitosa (green) and D. antarctica (purple) (H, B); D. antarctica (green) and D. flexuosa (purple) (C). Image after FISH with 45S (yellow) and 5S (red) rDNA (D). Chromosomal DAPI-staining–blue. Arrows point to the marker and B chromosomes. Scale bar—5 μm.