Fig 6. NILR1 is localised in plasma membrane.
(A) Confocal microscopy of epidermis of Nicotianna benthamiana transiently expressing 35S:NILR1-GFP and plasma membrane marker 35S:PIP2A-mCherry. Scale, 50 μm. (B-E) Leaf discs from tomato (B), N. benthamiana (C), sugarbeet (D) and rice plants were treated with water, HsNemaWater or flg22 and ROS burst was measured using L-012 based assay from 0 to 120 min. Bars represent mean ± SE for three technical replicates. Experiment was repeated three times with same results. RLU, relative light units.