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Each cell line (EAhy926, HL60, NB4, and U937) was treated with IDR or Am80 at 37°C for 4 h. Total RNA was extracted and analyzed by RT-PCR for 28 cycles. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA was used as a loading control. Relative signal intensity was determined. Data are the mean ± SD (n = 6). Significant differences are indicated by (*) when p < 0.05 compared with 0 μM.
