Figure 2. AKT phosphorylation of FOXO1 at serine 319 is critical for FOXO1 binding to IQGAP1.

• A
  Western blot analysis of LNCaP whole‐cell lysates (WCL) and co‐IP samples. Cell lysates were treated with or without λ phosphatase before IP.
• B
  Western blot analysis of WCL and co‐IP samples in DU145 cells 24 h after transfection with indicated plasmids.
• C
  Western blot analysis of WCL and co‐IP samples in DU145 cells 48 h after transfection with indicated siRNAs.
• D
  Western blot analysis of WCL and co‐IP samples in LNCaP cells. Cells were treated with 30 μM of LY294002 for 6 h before IP.
• E, F
  Western blot analysis of WCL and co‐IP samples in LNCaP cells 24 h after transfection with indicated plasmids.