Figure 3. AKT‐phosphorylated FOXO1 binds to IQGAP1 and inhibits IQGAP1 interaction with Raf, MEK, and ERK proteins.

• A
  Schematic diagram depicting the domain structure of IQGAP1 and 6 GST‐IQGAP1 constructs. CC, coiled‐coil domain.
• B
  LNCaP whole‐cell lysates (WCL) were subjected to GST pull‐down assay by GST or GST‐IQGAP1 recombinant proteins and Western blot analysis of FOXO1 proteins. Arrows indicate the proteins in expected molecular weight.
• C
  Western blot analysis of WCL and co‐IP samples in LNCaP cells 48 h after infection with lentivirus expressing control or FOXO1‐specific shRNA.
• D–F
  Western blot analysis of WCL and co‐IP samples in LNCaP cells 24 h after transfection with indicated plasmids. E.V., empty vector.