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. 2017 Mar 20;36(8):1100–1116. doi: 10.15252/embj.201696315

Figure 1. Efficient localization of HyD‐LIRs‐GFP to mRFP‐LC3B‐positive autophagosomes.

Figure 1

  • A
    Schematic model of the development of new autophagosome sensors. LIR: LC3‐interacting region, HyD: hydrophobic domain.
  • B, C
    Confocal images showing cellular localization of various LIR‐GFP constructs (HyD‐GFP, LIR(p62)‐GFP, HyD‐LIR(p62)‐GFP, LIR(Fy)‐GFP, or HyD‐LIR(Fy)‐GFP) together with mRFP‐LC3B in MEFs incubated with 100 nM rapamycin (Rapa) + 10 mM NH4Cl for 4 h. Scale bar: 10 μm.
  • D, E
    The bar graphs illustrate the ratios of sensor‐positive mRFP‐LC3B spots (D) and ratios of autophagosomal/cytosol (A/C) fluorescence intensity (E) of each tested GFP sensor construct. The values are presented as the mean ± SEM. ***P < 0.001, two‐tailed unpaired Student's t‐test. **P < 0.001, two‐tailed Mann–Whitney U‐test. The numbers on the bars indicate the number of cells used for the experiment.