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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Hepatology. 2017 Mar 3;65(4):1336–1351. doi: 10.1002/hep.29078

Figure 3. Hepatocyte death up-regulates genes associated with myeloid cell recruitment, predominantly in hepatocytes.

Figure 3

Two weeks following rAAV injection, C57BL/6J mice received DT and were sacrificed 6, 12, 24, 48, 72, or 96 hours later. (A) Fold change gene expression in total liver, hepatocytes, LSECs, and KCs compared to the No DT group median, Hprt used as reference gene. Data are median and range. (B) mRNA expression relative to Hprt in total liver, hepatocytes, LSECs, and KCs. Median values only represented. For Ccl7, non-detect values represented on graph are for hepatocyte population only. (A, B) Data are combined from two experiments, each data point represents an individual mouse (n = 4–6 per group). (A) Significance determined by a Kruskal-Wallis test followed by Dunn’s post-test of each time point to the No DT group. * represents significance compared to No Dt group; *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001; non-significance represented by absence of asterisk. DT, diphtheria toxin; Hepa, hepatocyte; KC, Kupffer cell; and LSEC, liver sinusoidal endothelial cell; and ND, non-detect.