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. 2017 Apr 15;28(8):1011–1020. doi: 10.1091/mbc.E16-05-0324

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© 2017 Botchkarev et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Cdc5 colocalizes at SPBs with Spc29 in early mitosis but with Spc72 only in cells with a long mitotic spindle. (A) Representative images of Cdc5-GFP localization relative to Spc29-RFP and Spc72-mCherry during mitosis in cells cultured asynchronously. (B) Calculation used to determine whether Cdc5 localizes to the nuclear or cytoplasmic SPB surface. (C) Quantified results of experiment in A using calculation in B. (D) Cdc5-GFP colocalizes with SPB component Spc42-RFP (arrows) but not with kinetochore component Nuf2-mCherry (asterisks) during early mitosis.