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. 2017 Apr 15;28(8):1011–1020. doi: 10.1091/mbc.E16-05-0324

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© 2017 Botchkarev et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Cdc14 is sufficient to release Cdc5 from its metaphase SPB population. (A) Cdc5-GFP fluorescence signal is lost in nocodazole-arrested wild-type cells after 2 h of Gal-Cdc14 overexpression. (B) Bfa1-GFP signal is retained in nocodazole-arrested cells after 2 h of Gal-Cdc14 overexpression. (C) Cdc5-GFP signal is lost from SPBs but not from the nucleus in both cdh1∆ and cdh1∆ bfa1∆ nocodazole-arrested cells after 2 h of Gal-Cdc14 overexpression. Arrows depict nuclei with Cdc5-GFP in the nucleus but not at SPBs. (D) Quantification of C (n > 100 of each strain at time zero; n > 50 of each strain at 2 h; repeated in triplicate).