FIGURE 5:

Cdc5 is retained in its Bfa1-independent population in FEAR mutants slk19∆ and spo12∆. (A) Cdc5-GFP can localize to both the mSPB and dSPB of asynchronously cycling anaphase slk19∆ and spo12∆ cells (wild-type n = 130; slk19∆ n = 164; spo12∆ n = 97). (B) Bfa1-GFP localizes asymmetrically to the dSPB in late anaphase slk19∆ cells (wild-type n = 123; slk19∆ n = 85). (C) Quantified analysis of the Cdc5-GFP SPB localization pattern at SPBs in wild-type (n = 96), bfa1∆ (n = 81), slk19∆ (n = 198), and slk19∆ bfa1∆ (n = 159) late anaphase cells. All experiments were performed in triplicate.