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. 2017 Apr 15;28(8):1021–1033. doi: 10.1091/mbc.E16-06-0461

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© 2017 Marchenko et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Analysis of filopodia motility with automated tracking software FiloTracker. (A) Phase-contrast image of hippocampal pyramidal neuron in culture, DIV5, with dendrites covered with filopodia; scale bar, 10 µm. (B) High-magnification image of boxed area in A during length tracking process in FiloTracker; red dotted line is a length estimate for the current frame; scale bar, 2 µm. (C) Output sample of length measurements from the filopodia in B (red); acquisition rate, 1 frame/s; periodicity of length, ∼122 ± 19 s. (D) Comparison of motile fraction of all filopodia in imaging session (t = 30 min) at DIV4–9 and DIV8–10 (t test, p < 0.05).