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. 2017 Apr 15;28(8):1088–1100. doi: 10.1091/mbc.E16-04-0214

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© 2017 Lapierre et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 10: — Growth of the Eps15-knockdown MDCK cell line in Matrigel resulted in multilumen cysts. The MDCK control knockdown cell line (top) and the MDCK Eps15 knockdown cell line (bottom) were grown in Matrigel for 2 d (top) or 7 d (bottom), fixed, and stained for Eps15 (green in merge), podocalyxin (red in merge), F-actin (cyan in merge), and DAPI (blue in merge). Bars, 5 μm. Numbers in the lower right corner of the merged images indicate the percentage of cysts exhibiting the illustrated morphology; 15 cysts from three separate experiments were counted.