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. 2017 Apr 15;28(8):1111–1122. doi: 10.1091/mbc.E16-07-0499

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© 2017 Iaea et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Microinjected HPCD and STARD4 overexpression enhances bidirectional sterol transport. HPCD and rhodamine–dextran were microinjected immediately before labeling of cells with DHE. U2OS-SRA cells were transfected with GFP-STARD4 for 18 h before the experiment. (A) FRAP measurements for single cells microinjected with HPCD or transfected with GFP-STARD4. Control, t_1/2 = 14.5 ± 1.5 min; HPCD (1 × 10⁹ molecules/cell), t_1/2 = 1.3 ± 0.3 min; GFP-STARD4, t_1/2 = 4.3 ± 0.5 min. (B) DHE efflux curves for cells microinjected with HPCD or transfected with GFP-STARD4. Control, t_1/2 = 14.9 ± 0.9 min; HPCD (0.5 × 10⁹ molecules/cell), t_1/2 = 4.1 ± 0.4 min; GFP-STARD4, t_1/2 = 4.9 ± 0.6 min. Each data point is derived from an average of at least 10 experiments (±SE). Data are fitted to single-exponential curves.